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- Title
TGF-β activates genes identified by differential mRNA display in pancreatic rudiments.
- Authors
Battelino, Tadej; Miralles, Francisco; Kr&zbreve;iˇnik, Ciril; Scharfmann, Raphaël; Czernichow, Paul
- Abstract
The effect of TGF-β on gene activation in embryonic pancreatic rudiments was investigated using differential mRNA display. Several cDNA bands were augmented and some were suppressed in the presence of TGF-β. Differentially expressed cDNAs were re-amplified, sequenced, and sequences compared to the GeneBank database. Glucagone and brain α-tropomyosin cDNAs were identified from the group of augmented cDNAs, and B-carboxypeptidase form the group of suppressed cDNAs. PCR experiments were confirmed with Northern blots. Obtained results are in accordance with immunohistochemical findings and render differential mRNA display a useful technique in identifying differentially expressed genes in embryonic pancreatic rudiments. Several unknown differentially expressed cDNA sequences obtained in our experiments remain to be identified.
- Subjects
GENETICS; PANCREATIC duct; EMBRYOLOGY; MESSENGER RNA; NUCLEOTIDE sequence; CARBOXYPEPTIDASES; IMMUNOHISTOCHEMISTRY
- Publication
Pflügers Archiv: European Journal of Physiology, 2000, Vol 439, Issue 7, pr026
- ISSN
0031-6768
- Publication type
Article
- DOI
10.1007/BF03376510