We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
A Ferrofluid with Surface Modified Nanoparticles for Magnetic Hyperthermia and High ROS Production.
- Authors
Cervantes, Oscar; Lopez, Zaira del Rocio; Casillas, Norberto; Knauth, Peter; Checa, Nayeli; Cholico, Francisco Apolinar; Hernandez-Gutiérrez, Rodolfo; Quintero, Luis Hector; Paz, Jose Avila; Cano, Mario Eduardo
- Abstract
A ferrofluid with 1,2-Benzenediol-coated iron oxide nanoparticles was synthesized and physicochemically analyzed. This colloidal system was prepared following the typical co-precipitation method, and superparamagnetic nanoparticles of 13.5 nm average diameter, 34 emu/g of magnetic saturation, and 285 K of blocking temperature were obtained. Additionally, the zeta potential showed a suitable colloidal stability for cancer therapy assays and the magneto-calorimetric trails determined a high power absorption density. In addition, the oxidative capability of the ferrofluid was corroborated by performing the Fenton reaction with methylene blue (MB) dissolved in water, where the ferrofluid was suitable for producing reactive oxygen species (ROS), and surprisingly a strong degradation of MB was also observed when it was combined with H2O2. The intracellular ROS production was qualitatively corroborated using the HT-29 human cell line, by detecting the fluorescent rise induced in 2,7-dichlorofluorescein diacetate. In other experiments, cell metabolic activity was measured, and no toxicity was observed, even with concentrations of up to 4 mg/mL of magnetic nanoparticles (MNPs). When the cells were treated with magnetic hyperthermia, 80% of cells were dead at 43 °C using 3 mg/mL of MNPs and applying a magnetic field of 530 kHz with 20 kA/m amplitude.
- Subjects
MAGNETIC nanoparticles; IRON oxide nanoparticles; COLLOIDS; FEVER; METHYLENE blue; REACTIVE oxygen species; HEAT stroke
- Publication
Molecules, 2022, Vol 27, Issue 2, p544
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules27020544