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- Title
Porcine teschovirus 2 induces an incomplete autophagic response in PK-15 cells.
- Authors
Gu, Yuanxing; Zhou, Yingshan; Shi, Xinfeng; Xin, Yongping; Shan, Ying; Chen, Cong; Cao, Tong; Fang, Weihuan; Li, Xiaoliang
- Abstract
Autophagy is a homeostatic process that has been shown to be vital in the innate immune defense against pathogens. However, little is known about the regulatory role of autophagy in porcine teschovirus 2 (PTV-2) replication. In this study, we found that PTV-2 infection induces a strong increase in GFP-LC3 punctae and endogenous LC3 lipidation. However, PTV-2 infection did not enhance autophagic protein degradation. When cellular autophagy was pharmacologically inhibited by wortmannin or 3-methyladenine, PTV-2 replication increased. The increase in virus yield via autophagy inhibition was further confirmed by silencing <italic>atg5</italic>, which is required for autophagy. Furthermore, PTV-2 replication was suppressed when autophagy was activated by rapamycin. Together, the results suggest that PTV-2 infection activates incomplete autophagy and that autophagy then inhibits further PTV-2 replication.
- Subjects
PORCINE somatotropin; PORCINE epidemic diarrhea virus; AUTOPHAGY; SWINE growth; ANIMAL genetics
- Publication
Archives of Virology, 2018, Vol 163, Issue 3, p623
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-017-3652-2