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- Title
Efficient foreign gene expression in planta using a plantago asiatica mosaic virus-based vector achieved by the strong RNA-silencing suppressor activity of TGBp1.
- Authors
Minato, Nami; Komatsu, Ken; Okano, Yukari; Maejima, Kensaku; Ozeki, Johji; Senshu, Hiroko; Takahashi, Shuichiro; Yamaji, Yasuyuki; Namba, Shigetou
- Abstract
Plant virus expression vectors provide a powerful tool for basic research as well as for practical applications. Here, we report the construction of an expression vector based on plantago asiatica mosaic virus (PlAMV), a member of the genus Potexvirus. Modification of a vector to enhance the expression of a foreign gene, combined with the use of the foot-and-mouth disease virus 2A peptide, allowed efficient expression of the foreign gene in two model plant species, Arabidopsis thaliana and Nicotiana benthamiana. Comparison with the widely used potato virus X (PVX) vector demonstrated that the PlAMV vector retains an inserted foreign gene for a longer period than PVX. Moreover, our results showed that the GFP expression construct PlAMV-GFP exhibits stronger RNA silencing suppression activity than PVX-GFP, which is likely to contribute to the stability of the PlAMV vector.
- Subjects
GENE expression in viruses; PLANTAGO; MOSAIC viruses; PLANT viruses; GENE silencing; FOOT &; mouth disease; POTATO virus X; VIRUSES
- Publication
Archives of Virology, 2014, Vol 159, Issue 5, p885
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-013-1860-y