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- Title
Features of the Population of Mouse Peritoneal Macrophages Isolated after Stimulation with Concanavalin A and Thioglycolate.
- Authors
Zubkova, E. S.; Dergilev, K. V.; Beloglazova, I. B.; Molokotina, Yu. D.; Boldyreva, M. A.; Tsokolaeva, Z. I.; Stafeev, I. S.; Menshikov, M. Yu.; Parfyonova, Ye. V.
- Abstract
Murine peritoneal macrophages isolated from the lavage fluid after administration of thioglycolate and concanavalin A are presented by two populations of cells of different diameters. Polarization of macrophages into a proinflammatory (M1) phenotype is accompanied by an increase in number of small cells. Macrophages obtained after administration of thioglycolate demonstrate higher tendency to anti-inflammatory (M2) phenotype, while macrophages isolated after administration of concanavalin A are committed in the proinflammatory direction. Lactate level is increased in M1 macrophages in comparison with M2 cells, which indicates predominance of glycolytic metabolism. Macrophages obtained after administration of concanavalin A have reduced mitochondrial potential, which reflects a tendency to apoptosis. Autophagy activation and inhibition neutralize the differences in pro- and anti-inflammatory properties of polarized macrophages obtained after thioglycolate administration, but have less pronounced effect on macrophages obtained after administration concanavalin A. Autophagy inhibitor increases mitochondrial potential in non-polarized macrophages obtained after administration of concanavalin A. These results demonstrate divergent properties of macrophages obtained after administration of glycolate and concanavalin A due to the difference in the mechanisms of experimental peritonitis.
- Subjects
CONCANAVALIN A; THIOGLYCOLIC acid; CELL populations; MACROPHAGES; MICE; PERITONEAL macrophages
- Publication
Bulletin of Experimental Biology & Medicine, 2021, Vol 171, Issue 4, p532
- ISSN
0007-4888
- Publication type
Article
- DOI
10.1007/s10517-021-05265-6