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- Title
Effects of different light qualities and plant growth regulators on the growth and secondary metabolite content of Lonicera macranthoides seedlings.
- Authors
Wu, Peiyin; Wang, Guiyuan; Cao, Zhengyan; Liu, Yanni; Xia, Ning; Wang, Qiyao; Si, Shuo; Shen, Xinlong; Yao, Yingge; Tang, Ning; Xu, Feng; Chen, Zexiong
- Abstract
Lonicera macranthoides, a medicinal herb mainly distributed in southern China, is widely utilized in traditional Chinese medicine for its high yield and strong pharmacological properties rich in phenolic acids and flavonoids. However, the factors regulating plant growth and secondary metabolism in L. macranthoides remain largely unknown. In this study, the effects of seven light qualities and plant growth regulators combinations on seedlings growth and metabolite accumulation were investigated. Plants under red light produced the greatest shoot and root biomass, and promoted plant photosynthesis, as indicated by significantly greater values of net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci), and transpiration rate (Tr), followed by white and blue light. The plant height and stem diameter of the tissue-cultured seedlings on the medium with 1.0 mg/L 6-benzyladenine (BA) + 1.0 mg/L indole 3-butyric acid (IBA) were the highest, while the seedlings on the medium containing 2.0 mg/L BA + 0.2 mg/L IBA produced the highest amounts of phenolic acids and flavonoids. Red light dramatically enhanced the accumulation of chlorogenic acid (CGA), ferulic acid, luteoloside, and rutin compared to those under other light spectrum conditions on media with low levels of auxin (0.2 mg/L IBA). However, higher auxin levels (1.0 mg/L IBA) dramatically inhibited CGA production under red light. Moreover, blue light promoted the accumulation of chlorogenic acid and luteoloside. The results indicate that the plant growth and secondary metabolism were dramatically influenced by light quality and auxin/cytokinin combinations. Our findings provide effective strategies aiming to enhance biomass yield and bioactive compound production in L. macranthoides.
- Subjects
CHINA; PLANT regulators; GROWTH regulators; PLANT metabolites; HONEYSUCKLES; BUTYRIC acid; CHLOROGENIC acid; CHINESE medicine; SEEDLINGS
- Publication
In Vitro Cellular & Developmental Biology Plant, 2023, Vol 59, Issue 5, p536
- ISSN
1054-5476
- Publication type
Article
- DOI
10.1007/s11627-023-10359-z