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- Title
Phenotypes of the ovarian follicular basal lamina predict developmental competence of oocytes.
- Authors
Irving-Rodgers, Helen F; Morris, Stephanie; Collett, Rachael A; Peura, Teija T; Davy, Margaret; Thompson, Jeremy G; Mason, Helen D; Rodgers, Raymond J
- Abstract
<bold>Background: </bold>The ovarian follicular basal lamina underlies the epithelial membrana granulosa and maintains the avascular intra-follicular compartment. Additional layers of basal lamina occur in a number of pathologies, including pili annulati and diabetes. We previously found additional layers of follicular basal lamina in a significant percentage of healthy bovine follicles. We wished to determine if this phenomenon existed in humans, and if it was related to oocyte function in the bovine.<bold>Methods and Results: </bold>We examined follicles from human ovaries (n = 18) by electron microscopy and found that many follicles had additional layers of basal lamina. Oocytes (n = 222) from bovine follicles with normal or unusual basal laminas were isolated and their ability to undergo in vitro maturation, fertilization and culture to blastocyst was compared. Healthy bovine follicles with a single layer of basal lamina had oocytes with significantly (P < 0.01) greater developmental competence than healthy follicles with additional layers of follicular basal lamina (65% versus 28%).<bold>Conclusions: </bold>These findings provide direct evidence that the phenotype of the follicular basal lamina is related to oocyte competence.
- Subjects
ANIMAL experimentation; BLASTOCYST; CATTLE; COMPARATIVE studies; ELECTRON microscopy; HUMAN reproductive technology; IMMUNITY; RESEARCH methodology; MEDICAL cooperation; BASAL lamina; OVARIES; OVUM; RESEARCH; PHENOTYPES; CYTOMETRY; EVALUATION research; IN vitro studies
- Publication
Human Reproduction, 2009, Vol 24, Issue 4, p936
- ISSN
0268-1161
- Publication type
journal article
- DOI
10.1093/humrep/den447