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- Title
EFFECT OF FLUOROQUINOLONES TREATMENT ON CERTAIN SPERM FUNCTION PARAMETERS AND SPERM DNA DENATURATION IN RATS: AN EXPERIMENTAL MODEL FOR MEN.
- Authors
Al-Dujaily, Saad S.; Hameed, Abdul-Kareem; Al-Saray, Dalia A.; Al-Ahmad, Hazim Esmaeel
- Abstract
Fluoroquinolones are excessively prescribed in the therapy of genital tract infections. However, fluoroquinolone treatment may be associated with fertility problems, insufficient information concerning their effect on fertility are present. The goal of this study is to elucidate the effects of two fluoroquinolones treatment i.e. ciprofloxacin and levofloxacin on certain sperm function parameters and even on reproductive tissues and to investigate whether these two antibiotics can affect sperm DNA integrity or chromatin quality. Eighty mature Albino male rats adult were enrolled in this study. The animals were randomly divided into 10 groups (8 animals in each). Four groups treated with levofloxacin with a dose of 37.5 mg/kg/day and 75 mg/kg/day of levofloxacin and four ciprofloxacin treated groups which were treated with a dose of 40mg/kg/day and 80 mg/kg/ day of ciprofloxacin and two control groups, for each treatment dose the treatment continue for either 14 days or 28 days. Certain epididymal sperm function parameters namely : sperm concentration, sperm motility and morphologically normal sperm percentage were analyzed. In addition to analysis of sperm DNA integrity and chromatin quality, histopathology of testes and epididymus were examined. The results shown that certain sperm function parameters were not significantly affected when levofloxacin and ciprofloxacin were administered orally for 14 days. While, a significant reduction in; sperm concentration, percentage of morphologically normal sperm and sperm motility were observed when the two medicines were administered for 28 days. A significant increase in the level of DNA fragmentation with a significant reduction in chromatin quality were also observed in fluoroquinolone treated groups following 28 days treatment. The testes of rat treated with fluoroquinolones showed changes in the number of spermatozoa in some seminiferous and epididymal tubules when the two medicine were applied in high doses and for 28 days. These results indicate that fluoroquinolone treatment can be used for 14 days in rats (which equal 28 days in men) with no harmful effects on certain sperm parameters and adversely affect fertility potential in male in a dose and time dependent manner mainly by interfering with sperm DNA integrity level
- Subjects
FLUOROQUINOLONES; QUINOLONE antibacterial agents; SPERMATOZOA; DNA denaturation; CIPROFLOXACIN
- Publication
Biochemical & Cellular Archives, 2021, Vol 21, Issue 1, p477
- ISSN
0972-5075
- Publication type
Article