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- Title
超高效液相色谱-串联质谱法检测 小菜蛾血清中的β-蜕皮激素.
- Authors
孙诗晴; 王念猛; 薛超彬
- Abstract
[Objectives] A method for using UPLC-MS/MS to detect β-ecdysone in the serum of Plutella xylostella was developed. [Methods] Serum β-ecdysone of P. xylostella was extracted with acetonitrile and precipitated with protein. It was then separated in a C18 column, analyzed with UPLC-MS/MS and the β-ecdysone content determined using the internal standard brassinolide. [Results] The results show that the linear correlation coefficient of β-ecdysone was R2 = 0.999 8 in the range of 0.5-50 μg/L, the average recovery was 91.9%-104.9% at levels of 0.5 μg/L, 10 μg/L and 50 μg/L, the relative standard deviation (RSDs, n = 5) was 3.2%-9.2% and the quantitative limit of the method was 0.5 μg/L. [Conclusion] This method has the advantages of being relatively simple, sensitive, stable and applicable. It should provide an accurate method for the detection and measurement of β-ecdysone in the serum of other insects.
- Publication
Chinese Journal of Applied Entomology, 2020, Vol 57, Issue 2, p460
- ISSN
2095-1353
- Publication type
Article
- DOI
10.7679/j.issn.2095-1353.2020.052