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- Title
Metagenomic and metatranscriptomic analysis of saliva reveals disease-associated microbiota in patients with periodontitis and dental caries.
- Authors
Belstrøm, Daniel; Constancias, Florentin; Liu, Yang; Yang, Liang; Drautz-Moses, Daniela I.; Schuster, Stephan C.; Kohli, Gurjeet Singh; Jakobsen, Tim Holm; Holmstrup, Palle; Givskov, Michael
- Abstract
The taxonomic composition of the salivary microbiota has been reported to differentiate between oral health and disease. However, information on bacterial activity and gene expression of the salivary microbiota is limited. The purpose of this study was to perform metagenomic and metatranscriptomic characterization of the salivary microbiota and test the hypothesis that salivary microbial presence and activity could be an indicator of the oral health status. Stimulated saliva samples were collected from 30 individuals (periodontitis: n = 10, dental caries: n = 10, oral health: n = 10). Salivary microbiota was characterized using metagenomics and metatranscriptomics in order to compare community composition and the gene expression between the three groups. Streptococcus was the predominant bacterial genus constituting approx. 25 and 50% of all DNA and RNA reads, respectively. A significant disease-associated higher relative abundance of traditional periodontal pathogens such as Porphyromonas gingivalis and Filifactor alocis and salivary microbial activity of F. alocis was associated with periodontitis. Significantly higher relative abundance of caries-associated bacteria such as Streptococcus mutans and Lactobacillus fermentum was identified in saliva from patients with dental caries. Multiple genes involved in carbohydrate metabolism were significantly more expressed in healthy controls compared to periodontitis patients. Using metagenomics and metatranscriptomics we show that relative abundance of specific oral bacterial species and bacterial gene expression in saliva associates with periodontitis and dental caries. Further longitudinal studies are warranted to evaluate if screening of salivary microbial activity of specific oral bacterial species and metabolic gene expression can identify periodontitis and dental caries at preclinical stages. Oral health: Signals from gene activity in saliva Genetic analysis of saliva reveals the activity of bacteria linked to gum disease and tooth decay and may prove useful in early diagnosis. Daniel Belstrøm and colleagues at the University of Copenhagen, Denmark, with co-workers at Nanyang Technological University in Singapore, analyzed saliva from 10 patients with periodontitis gum disease, 10 with dental caries and 10 with good oral health. DNA analysis revealed which bacteria were present, while examining RNA revealed which bacterial genes were most active. The procedure identified greater abundance and activity of bacteria linked to each specific oral condition in the oral disease groups, and also found distinctive bacterial activity in those people with good oral health. Further studies should investigate the possibility of testing bacterial gene activity in saliva to identify oral diseases before they become clinically evident.
- Publication
NPJ Biofilms & Microbiomes, 2017, Vol 3, Issue 1, pN.PAG
- ISSN
2055-5008
- Publication type
Article
- DOI
10.1038/s41522-017-0031-4