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- Title
Confirmation of a Major QTL influencing Oral Morphine intake in C57 and DBA Mice Using Reciprocal Congenic Strains.
- Authors
Ferraro, Thomas N.; Gregory T. Golden; Smith, George G.; Martin, James F.; Schwebel, Candice L.; Doyle, Glenn A.; Buono, Russell J.; Berrettini, Wade H.
- Abstract
CS7BLJ6 (B6) and DBA/2 (D2) mice exhibit disparate behavior when tested for voluntary morphine intake in a two-bottle choice drinking paradigm with B6 mice consuming 10 times more drug than D2 mice. Previous genetic mapping studies identified a locus, Mop2, on the proximal part of chromosome I 0 that explained over half of the genetic variance in this mouse model of opioid self- administration. We constructed a set of reciprocal congenic strains between 86 and D2 mice in which the proximal portion of chromosome I 0 has been introgressed from one strain onto the background of the other. We tested mice from this pair of reciprocal strains together with progenitor B6 and D2 mice in a two-bottle choice drinking paradigm with morphine and quinine. The results showed that introgression of chromosome JO alleles from the B6 strain onto a D2 genetic background increased voluntary morphine intake four-fold compared to progenitor D2 mice. Preference for morphine was also increased significantly in D2.B6-Mop2 mice compared to progenitor D2 mice. Conversely, introgression of chromosome 10 alleles from the D2 strain onto a 86 genetic background decreased morphine intake by half compared to progenitor B6 mice in 86.D2 -Mop2 mice; however, high morphine preference was maintained in this congenic strain most likely due to strong quinine aversion. When quinine was eliminated from the control bottle, morphine preference in B6.D2-Mop2 mice was decreased significantly relative to B6 and D2.B6-Mop2 mice. Overall, these data confirm the existence of a gene(s) on chromosome 10 proximal to D10Mit124 that has a strong influence on the difference in morphine drinking behavior between 86 and 02 mice. - I I .
- Subjects
MORPHINE; NARCOTICS; QUININE; MICE; OPIOIDS; PSYCHIATRIC drugs
- Publication
Neuropsychopharmacology, 2005, Vol 30, Issue 4, p742
- ISSN
0893-133X
- Publication type
Article
- DOI
10.1038/sj.npp.1300592