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- Title
Obese and lean porcine difference of FoxO1 and its regulation through C/EBPβ and PI3K/GSK3β signaling pathway.
- Authors
Pang, W. J.; Wei, N.; Wang, Y.; Xiong, Y.; Chen, F. F.; Wu, W. J.; Zhao, C. Z.; Sun, S. D.; Yang, G. S.
- Abstract
Forkhead box O 1 (FoxOl) is an important transcription factor implicated in adipogenesis. In this study, we detected the breed differences in FoxOl between Bamei pigs (an obese breed) and Large White pigs (a lean breed). Compared with Large White pigs, the BW of Bamei pigs was lower (P < 0.01), but back fat thickness, fat percent, and intramuscular fat content were greater (P < 0.01). The levels of FoxOl mRNA and protein were lower (P < 0.01) in subcutaneous adipose tissue (SAT) of Bamei pigs at 180 d, adipocytes and stromal-vascular fraction extracted from SAT of Bamei pigs at 1 d compared with Large White pigs. Knockdown of FoxOl increased triglyceride content (P < 0.01) and upregulated the levels of adipocyte fatty-acid binding protein, PPARγ, and CCAAT enhancer binding protein a (C/EBPα) at 6 d after porcine preadipocytes were induced. Furthermore, the transcriptional regulation of FoxOl through C/EBPβ during early porcine preadipocyte differentiation and the effect of insulin on phosphoinositide 3 kinase (PI3K)/ glycogen synthase kinase 3β (GSK3β) signal pathway by FoxOl were examined. The results indicated that FoxOl inhibited transcription activity of C/EBPβ, whereas C/ EBPβ did not affect transcription activity of FoxOl. At 6 and 12 h of early differentiation, knockdown of FoxOl triggered the transcription activity of C/EBPβ. In addition, FoxOl protein interacted with C/EBPβ protein in porcine adipocytes at 12 h after induction. Under treatment with 100 nM insulin, knockdown or overexpression of FoxOl mediated PI3K/GSK3β signaling via upregulating or downregulating the levels of GSK3β and its phosphorylation in adipocytes. Taken together, there is low, but detectable, expression ofFoxOl in SAT of obese pigs and FoxOl inhibited adipogenesis through C/EBPβ and PI3K/GSK3β signaling pathway. These findings provide useful information to further the understanding of the function of FoxOl in porcine adipogenesis.
- Subjects
ADIPOSE tissues; PROTEINS; TRANSCRIPTION factors; ADIPOGENESIS; FAT cells
- Publication
Journal of Animal Science, 2014, Vol 92, Issue 5, p1968
- ISSN
0021-8812
- Publication type
Article
- DOI
10.2527/jas.2013-7098