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- Title
Comparison of modern analyzers for the detection of antiphospholipid antibodies in patients with SLE.
- Authors
Vossen, Diana; Hofbauer, Monika; Kersten, Jan F.; Krieger, Thorsten; Peine, Sven; Gutensohn, Kai
- Abstract
Background: The anti-cardiolipin and β2-glycoprotein I antibodies represent the important diagnostic parameters in routine autoimmune screening and represent the important diagnostic criteria for systemic lupus erythematosus (SLE). In this study, five different automated, semiautomated, and manual immunoassays detecting IgG/IgM anti-cardiolipin and anti-β2-glycoprotein I antibodies were tested. Methods: A total of 43 samples from the patients with SLE diagnosed in the rheumatology outpatient centers in Germany were included in this study. Results: For anticardiolipin and anti-β2-glycoprotein I, considerable differences in the percentage of the positive results were found between all the five methods, and the itemization of all the positive test results reached only a poor accordance. These findings were confirmed by Cohen's κ coefficients. Conclusions: We found a moderate to poor accordance between the five different modern test systems for anticardiolipin and anti-β2-glycoprotein I antibodies. Such discrepancies can result in the clinical misinterpretation of data and may lead to wrong diagnoses. Therefore, further standardization of the tests for the antiphospholipid antibodies (APAs) is required.
- Subjects
GERMANY; SYSTEMIC lupus erythematosus diagnosis; IMMUNOGLOBULINS; STATISTICS; DATA analysis software; DESCRIPTIVE statistics; AUTOANALYZERS
- Publication
Journal of Laboratory Medicine / Laboratoriums Medizin, 2013, Vol 37, Issue 3, p139
- ISSN
0342-3026
- Publication type
Journal Article
- DOI
10.1515/labmed-2012-0076