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- Title
Mitochondria-Targeted Curcumin: A Potent Antibacterial Agent against Methicillin-Resistant Staphylococcus aureus with a Possible Intracellular ROS Accumulation as the Mechanism of Action.
- Authors
Leferman, Carmen-Ecaterina; Stoica, Laura; Stoica, Bogdan Alexandru; Ciubotaru, Alin Dumitru; Badescu, Aida Corina; Bogdanici, Camelia-Margareta; Neagu, Tiberiu Paul; Ghiciuc, Cristina-Mihaela
- Abstract
Mitocurcumin (a triphenylphosphonium curcumin derivative) was previously reported as a selective antitumoral compound on different cellular lines, as well as a potent bactericidal candidate. In this study, the same compound showed strong antimicrobial efficacy against different strains of methicillin-resistant Staphylococcus aureus (MRSA). The minimum inhibitory concentration was identical for all tested strains (four strains of MRSA and one strain of methicillin-sensitive Staphylococcus aureus), suggesting a new mechanism of action compared with usual antibacterial agents. All tested strains showed a significant sensitivity in the low micromolar range for the curcumin-triphenylphosphonium derivative. This susceptibility was modulated by the menadione/glutathione addition (the addition of glutathione resulted in a significant increase in minimal inhibitory concentration from 1.95 to 3.9 uM, whereas adding menadione resulted in a decrease of 0.49 uM). The fluorescence microscopy showed a better intrabacterial accumulation for the new curcumin-triphenylphosphonium derivative compared with simple curcumin. The MitoTracker staining showed an accumulation of reactive oxygen species (ROS) for a S. pombe superoxide dismutase deleted model. All results suggest a new mechanism of action which is not influenced by the acquired resistance of MRSA. The most plausible mechanism is reactive oxygen species (ROS) overproduction after a massive intracellular accumulation of the curcumin-triphenylphosphonium derivative.
- Subjects
METHICILLIN-resistant staphylococcus aureus; ANTIBACTERIAL agents; CURCUMIN; REACTIVE oxygen species; STAPHYLOCOCCUS aureus
- Publication
Antibiotics (2079-6382), 2023, Vol 12, Issue 2, p401
- ISSN
2079-6382
- Publication type
Article
- DOI
10.3390/antibiotics12020401