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- Title
Validation of the GSP ® /DELFIA ® Anti-SARS-CoV-2 IgG Kit Using Dried Blood Samples for High-Throughput Serosurveillance and Standardized Quantitative Measurement of Anti-Spike S1 IgG Antibody Responses Post-Vaccination.
- Authors
Cicalini, Ilaria; Del Boccio, Piero; Zucchelli, Mirco; Rossi, Claudia; Natale, Luca; Demattia, Gianmaria; De Bellis, Domenico; Damiani, Verena; Tommolini, Maria Lucia; Pizzinato, Erika; Frisco, Alberto; Verrocchio, Sara; Bucci, Ines; Stuppia, Liborio; De Laurenzi, Vincenzo; Pieragostino, Damiana
- Abstract
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has caused a major global public health crisis. In response, researchers and pharmaceutical companies worked together for the rapid development of vaccines to reduce the morbidity and mortality associated with viral infection. Monitoring host immunity following virus infection and/or vaccination is essential to guide vaccination intervention policy. Humoral immune response to vaccination can be assessed with serologic testing, and indeed, many serological immunoassays are now in use. However, these many different assays make the standardization of test results difficult. Moreover, most published serological tests require venous blood sampling, which makes testing large numbers of people complex and costly. Here, we validate the GSP®/DELFIA® Anti-SARS-CoV-2 IgG kit using dried blood samples for high-throughput serosurveillance using standard quantitative measurements of anti-spike S1 IgG antibody concentrations. We then apply our validated assay to compare post-vaccination anti-SARS-CoV-2 S1 IgG levels from subjects who received a double dose of the AZD1222 vaccine with those vaccinated with a heterologous strategy, demonstrating how this assay is suitable for large-scale screening to achieve a clearer population immune picture.
- Subjects
SARS-CoV-2; ANTIBODY formation; BLOOD sampling; IMMUNOGLOBULIN G; HUMORAL immunity
- Publication
Vaccines, 2022, Vol 10, Issue 4, pN.PAG
- ISSN
2076-393X
- Publication type
Article
- DOI
10.3390/vaccines10040514