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- Title
Genetic incorporation of d-lysine into diketoreductase in Escherichia coli cells.
- Authors
Liu, Zhizhi; Yang, Xin; Yi, Denghuan; Wang, Shuzhen; Chen, Yijun
- Abstract
Pyrococcus horikoshii lysyl-tRNA synthetase/tRNA orthogonal pair exhibited high selectivity towards d-lysine in the presence of excess amount of d-lysine. Based on the observation, this orthogonal pair was employed to encode d-lysine, and d-lysine was site-specifically incorporated into the diketoreductase in E. coli cells. Graphical abstract: [Figure not available: see fulltext.]
- Subjects
LYSINE; ESCHERICHIA coli; PYROCOCCUS horikoshii; TRANSFER RNA; BACTERIAL cells; GENETIC code
- Publication
Amino Acids, 2012, Vol 43, Issue 6, p2553
- ISSN
0939-4451
- Publication type
Article
- DOI
10.1007/s00726-012-1311-1