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- Title
Therapeutic drug monitoring of lopinavir/ritonavir in pregnancy.
- Authors
Lambert, J. S.; Else, L. J.; Jackson, V.; Breiden, J.; Gibbons, S.; Dickinson, L.; Back, D. J.; Brennan, M.; Connor, E. O.; Boyle, N.; Fleming, C.; Coulter-Smith, S.; Khoo, S. H.
- Abstract
Objectives The aim of the study was to determine total and unbound lopinavir (LPV) plasma concentrations in HIV-infected pregnant women receiving lopinavir/ritonavir (LPV/r tablet) undergoing therapeutic drug monitoring (TDM) during pregnancy and postpartum. Methods Women were enrolled in the study who were receiving the LPV/r tablet as part of their routine prenatal care. Demographic and clinical data were collected and LPV plasma (total) and ultrafiltrate (unbound) concentrations were determined in the first, second and third trimesters using highperformance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Postpartum sampling was performed where applicable. Antepartum and postpartum trough concentrations (Ctrough) were compared independently [using analysis of variance (ANOVA)] and on a longitudinal basis (using a paired t-test). Results Forty-six women were enrolled in the study (38 Black African). Forty women initiated LPV/r treatment in pregnancy. Median (range) gestation at initiation was 25 (15-36) weeks and median (range) baseline CD4 count and viral load were 346 (14-836) cells/μL and 8724 (<50-267408) HIV-1 RNA copies/mL, respectively. Forty women (87%) had LPV concentrations above the accepted minimum effective concentration for wild-type virus (MEC; 1000 ng/mL). Geometric mean (95% confidence interval [CI]) total LPV concentrations in the first/second [3525 (2823-4227) ng/mL; n=16] and third [3346 (2813-3880) ng/mL; n=43] trimesters were significantly lower relative to postpartum [5136 (3693-6579) ng/mL; n=12] (P=0.006). In a paired analysis (n=512), LPV concentrations were reduced in the third trimester [3657 (2851-4463) ng/mL] vs. postpartum (P=0.021). No significant differences were observed in the LPV fraction unbound (fu%). Conclusions The above target concentrations achieved in the majority of women and similarities in the fu% suggest standard dosing of the LPV/r tablet is appropriate during pregnancy. However, reduced LPV concentrations in the second/third trimesters and potentially compromised adherence highlight the need for TDM-guided dose adjustment in certain cases.
- Subjects
IRELAND; THERAPEUTIC use of protease inhibitors; ACADEMIC medical centers; ANALYSIS of variance; ANTIVIRAL agents; BLOOD cell count; COMPUTER software; CONFIDENCE intervals; DRUG monitoring; HIV infections; MEDICAL cooperation; DURATION of pregnancy; RESEARCH; STATISTICAL hypothesis testing; STATISTICS; T cells; T-test (Statistics); DATA analysis; PROTEASE inhibitors; VIRAL load; DRUG dosage; PREGNANCY
- Publication
HIV Medicine, 2011, Vol 12, Issue 3, p166
- ISSN
1464-2662
- Publication type
Article
- DOI
10.1111/j.1468-1293.2010.00865.x