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- Title
Involvement of volume-sensitive Cl<sup>−</sup> channels in the proliferation of human subcutaneous pre-adipocytes.
- Authors
Hu, Hao; Li, Dong-Ling; Fan, Lin; Ren, Jun; Wang, Sheng-Peng; Jia, Bing; Liu, Bing-Hang; Sun, Lei; Yu, Xiao-Jiang; Zang, Wei-Jin
- Abstract
1. Obesity is a significant challenge in terms of public health and preventive medicine. Inhibition of pre-adipocyte proliferation is believed to be important in the proposed anti-obesity mechanism. The aim of the present study was to examine the interplay between Cl− channels and their possible involvement in the proliferation of undifferentiated human pre-adipocytes. 2. Pre-adipocytes were isolated from human abdominal subcutaneous adipose tissue. Membrane ion currents were recorded using the whole-cell patch-clamp technique. Expression of the Cl− channel ClC-3 gene and protein was determined by reverse transcription–polymerase chain reaction (RT-PCR) and western blot, respectively. Cell proliferation was evaluated using the [3H]-thymidine incorporation assay. 3. Electrophysiological recordings revealed a volume-sensitive Cl− current ( ICl.vol) expressed in pre-adipocytes that was activated under hyposmotic conditions (external osmolarity decreased by 80%) and inhibited by the Cl− channel blocker tamoxifen. 4. Expression of the ClC-3 channel gene and protein was confirmed by RT-PCR and western blot analysis. Blocking ICl.vol with tamoxifen supressed the proliferation of pre-adipocytes in a concentration-dependent manner. 5. Collectively, the results of the present study indicate that the volume-sensitive Cl− channel participates in regulation of the proliferation of human subcutaneous pre-adipocytes.
- Subjects
OBESITY; ETIOLOGY of diseases; FAT cells; PUBLIC health; TAMOXIFEN; CELL proliferation; CELL growth; ION channels
- Publication
Clinical & Experimental Pharmacology & Physiology, 2010, Vol 37, Issue 1, p29
- ISSN
0305-1870
- Publication type
Article
- DOI
10.1111/j.1440-1681.2009.05223.x