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- Title
Characterization of antisera to paired helical filaments and tau. Implication for the extent of tau tightly bound to paired helical filaments.
- Authors
Ihara, Y; Kondo, J; Miura, R; Nakagawa, Y; Mori, H; Honda, T
- Abstract
The two antisera to paired helical filaments (PHF) and tau, which stained neurofibrillary tangles similarly in tissue section, were characterized. Anti-PHF reacted exclusively with the carboxyl terminal portion of tau, whereas antitau reacted with the amino terminal portion. In the blots of PHF prepared in Sarkosyl (Sarkosyl-PHF), anti-PHF stained an extensive smear from the top to the bottom of the gel, while antitau did not stain this kind of smear but labeled a small amount of intact tau that was bound to Sarkosyl-PHF. An LDS- or SDS-insoluble residue of Sarkosyl-PHF was labeled with anti-PHF, but not with antitau. Antitau-defined epitopes were susceptible to protease, whereas anti-PHF-defined epitopes were unaffected. Based on these observations, we conclude that Sarkosyl-PHF were composed largely of the carboxyl terminal region of tau and accompanied by a small amount of intact tau on their surfaces.
- Subjects
IMMUNOGLOBULIN analysis; ALZHEIMER'S disease; AMINO acids; ANTIGENS; BLOOD; COMPARATIVE studies; DOCUMENTATION; ENZYME-linked immunosorbent assay; HIGH performance liquid chromatography; IMMUNOBLOTTING; RESEARCH methodology; MEDICAL cooperation; NERVE tissue proteins; NEURONS; RESEARCH; EVALUATION research; IN vitro studies; CHEMICAL inhibitors
- Publication
Gerontology, 1990, Vol 36, Issue 1, p15
- ISSN
0304-324X
- Publication type
journal article