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- Title
ER-mitochondria cross-talk is regulated by the Ca<sup>2+</sup> sensor NCS1 and is impaired in Wolfram syndrome.
- Authors
Angebault, Claire; Fauconnier, Jérémy; Patergnani, Simone; Rieusset, Jennifer; Danese, Alberto; Affortit, Corentin A.; Jagodzinska, Jolanta; Mégy, Camille; Quiles, Mélanie; Cazevieille, Chantal; Korchagina, Julia; Bonnet-Wersinger, Delphine; Milea, Dan; Hamel, Christian; Pinton, Paolo; Thiry, Marc; Lacampagne, Alain; Delprat, Benjamin; Delettre, Cécile
- Abstract
Communication between the endoplasmic reticulum (ER) and mitochondria plays a pivotal role in Ca2+ signaling, energy metabolism, and cell survival. Dysfunction in this cross-talk leads to metabolic and neurodegenerative diseases. Wolfram syndrome is a fatal neurodegenerative disease caused by mutations in the ER-resident protein WFS1. Here, we showed that WFS1 formed a complex with neuronal calcium sensor 1 (NCS1) and inositol 1,4,5-trisphosphate receptor (IP3R) to promote Ca2+ transfer between the ER and mitochondria. In addition, we found that NCS1 abundance was reduced in WFS1-null patient fibroblasts, which showed reduced ER-mitochondria interactions and Ca2+ exchange. Moreover, in WFS1-deficient cells, NCS1 overexpression not only restored ER-mitochondria interactions and Ca2+ transfer but also rescued mitochondrial dysfunction. Our results describe a key role of NCS1 in ER-mitochondria cross-talk, uncover a pathogenic mechanism for Wolfram syndrome, and potentially reveal insights into the pathogenesis of other neurodegenerative diseases.
- Subjects
ENDOPLASMIC reticulum; MITOCHONDRIA; ENERGY metabolism; NEURODEGENERATION; GENETIC mutation
- Publication
Science Signaling, 2018, Vol 11, Issue 553, p1
- ISSN
1945-0877
- Publication type
Article
- DOI
10.1126/scisignal.aaq1380