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- Title
Genomic Analysis of Wolbachia from Laodelphax striatellus (Delphacidae, Hemiptera) Reveals Insights into Its "Jekyll and Hyde" Mode of Infection Pattern.
- Authors
Bing, Xiao-Li; Zhao, Dian-Shu; Sun, Jing-Tao; Zhang, Kai-Jun; Hong, Xiao-Yue
- Abstract
Wolbachia is a widely distributed intracellular bacterial endosymbiont among invertebrates. The w StriCN, the Wolbachia strain that naturally infects an agricultural pest Laodelphax striatellus , has a "Jekyll and Hyde" mode of infection pattern with positive and negative effects: It not only kills many offspring by inducing cytoplasmic incompatibility (CI) but also significantly increases host fecundity. In this study, we assembled the draft genome of w StriCN and compared it with other Wolbachia genomes to look for clues to its Jekyll and Hyde characteristics. The assembled w StriCN draft genome is 1.79 Mb in size, which is the largest Wolbachia genome in supergroup B. Phylogenomic analysis showed that w StriCN is closest to Wolbachia from Asian citrus psyllid Diaphorina citri. These strains formed a monophylogentic clade within supergroup B. Compared with other Wolbachia genomes, w StriCN contains the most diverse insertion sequence families, the largest amount of prophage sequences, and the most ankyrin domain protein coding genes. The w StriCN genome encodes components of multiple secretion systems, including Types I, II, IV, VI, Sec, and Tac. We detected three pairs of homologs for CI factors CifA and CifB. These proteins harbor the catalytic domains responsible for CI phenotypes but are phylogenetically and structurally distinct from all known Cif proteins. The genome retains pathways for synthesizing biotin and riboflavin, which may explain the beneficial roles of w StriCN in its host planthoppers, which feed on nutrient-poor plant sap. Altogether, the genomic sequencing of w StriCN provides insight into understanding the phylogeny and biology of Wolbachia.
- Subjects
VITAMIN B2; WOLBACHIA; SAP (Plant); COMPARATIVE genomics; HEMIPTERA; CATALYTIC domains; CITRUS greening disease; PROTEIN domains
- Publication
Genome Biology & Evolution, 2020, Vol 12, Issue 2, p3818
- ISSN
1759-6653
- Publication type
Article
- DOI
10.1093/gbe/evaa006