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- Title
Comparison of Artery Organ Culture and Co-culture Models for Studying Endothelial Cell Migration and Its Effect on Smooth Muscle Cell Proliferation and Migration.
- Authors
YONG-UNG LEE; JIAN LUO; SPRAGUE, EUGENE; HAI-CHAO HAN
- Abstract
Arterial restenosis associated with intimal hyperplasia is the major cause of long-term failure of vascular interventions. Endothelium injury and the proliferation and migration of smooth muscle cells (SMC) are key events in the development of intimal hyperplasia. The objectives of this study were to develop an ex vivo artery injury model for studying endothelial cell (EC) migration and to compare it with an in vitro co-culture arterial wall injury model in terms of the effect of flow on EC migration and its effect on SMC migration and proliferation. Our results demonstrated that shear flow improves reendothelialization in the injured area by promoting EC migration. The migration distance of ECs is much smaller in the arteries than in an in vitro cell culture model (3.57 ± 1.29 mm vs. 5.2 ± 1.4 cm, p < 0.001). SMC proliferation was significantly less in the EC intact and reendothelialization areas than in the EC denuded areas indicating that reendothelialization suppresses SMC proliferation. Our models provide a new approach to study techniques to enhance endothelium healing.
- Subjects
ARTERIES; ORGAN culture; CELL migration; CELL proliferation; CELLULAR pathology; CELL culture
- Publication
Annals of Biomedical Engineering, 2010, Vol 38, Issue 3, p801
- ISSN
0090-6964
- Publication type
Article
- DOI
10.1007/s10439-009-9877-9