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- Title
Nitro-Oxidative Stress in a Mice Model of Toluene Diisocyanate -Induced Asthma.
- Authors
Persecă, Ovidiu; Orasăn, Remus; Cătoi, Cornel; Taulescu, Marian Aurel; PÂrvu, Alina Elena
- Abstract
Toluene-2,4-diisocyanate (TDI) induced occupational asthma mechanisms are not completely known. The aim of the study was to evaluate the nitro-oxidative stress associated to experimental mice TDI induced asthma. It was used a model of mice experimental exposure to TDI for 10 days. The study groups (n = 12) were: a negative control group with skin sensitization and intranasal challenge, performed with saline; group with sensitization and challenge with the solvent; group with sensitization and challenge with TDI; groups with TDI and aminoguanidine (AG) (50 mg/kg/d i.p.) or NG-nitro-L-arginine methyl ester (NAME), (5 mg/kg/d i.p.) or arginine (ARG) (100 mg/kg/d i.p.) or trolox (20 mg/ kg/ day ip). On the 11th day blood was collected by cardiac puncture, and nitro-oxidative stress was. assessed by total oxidative status (TOS), total antioxidative capacity (TAC), oxidative stress index (OSI) and serum nitrates and nitrites (NOx). TDI increased TOS, OSI and NOx and reduced TAC. The solvent did not influence any parameter. AG, NAME and Trolox reduced NOx, TOS and OSI. Experimental exposure of mice to TDI caused systemic nitro-oxidative stress, and NOx reduction and Trolox lowered the systemic nitro-oxidative stress
- Subjects
TOLUENE diisocyanate; OBSTRUCTIVE lung diseases; OXIDATIVE stress; ASTHMA; LABORATORY mice
- Publication
Bulletin of the University of Agricultural Sciences & Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2012, Vol 69, Issue 1/2, p177
- ISSN
1843-5270
- Publication type
Article