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- Title
Chemogenetic approaches to dissect the role of H<sub>2</sub>O<sub>2</sub> in redox-dependent pathways using genetically encoded biosensors.
- Authors
Zaki, Asal Ghaffari; Erdoğan, Yusuf C.; Caglar, Tuba Akgul; Eroglu, Emrah
- Abstract
Chemogenetic tools are recombinant enzymes that can be targeted to specific organelles and tissues. The provision or removal of the enzyme substrate permits control of its biochemical activities. Yeast-derived enzyme D-amino acid oxidase (DAAO) represents the first of its kind for a substrate-based chemogenetic approach to modulate H2O2 concentrations within cells. Combining these powerful enzymes with multiparametric imaging methods exploiting genetically encoded biosensors has opened new lines of investigations in life sciences. In recent years, the chemogenetic DAAO approach has proven beneficial to establish a new role for (patho)physiological oxidative stress on redoxdependent signaling and metabolic pathways in cultured cells and animal model systems. This mini-review covers established or emerging methods and assesses newer approaches exploiting chemogenetic tools combined with genetically encoded biosensors.
- Subjects
PHYSIOLOGICAL stress; LIFE sciences; CELLULAR signal transduction; ENZYMES
- Publication
Biochemical Society Transactions, 2022, Vol 50, Issue 1, p335
- ISSN
0300-5127
- Publication type
Article
- DOI
10.1042/BST20210506