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- Title
Interferon-γ Limits Diabetogenic CD8<sup>+</sup> T-Cell Effector Responses in Type 1 Diabetes.
- Authors
Driver, John P.; Racine, Jeremy J.; Cheng Ye; Lamont, Deanna J.; Newby, Brittney N.; Leeth, Caroline M.; Chapman, Harold D.; Brusko, Todd M.; Yi-Guang Chen; Mathews, Clayton E.; Serreze, David V.; Ye, Cheng; Chen, Yi-Guang
- Abstract
Type 1 diabetes development in the NOD mouse model is widely reported to be dependent on high-level production by autoreactive CD4+ and CD8+ T cells of interferon-γ (IFN-γ), generally considered a proinflammatory cytokine. However, IFN-γ can also participate in tolerance-induction pathways, indicating it is not solely proinflammatory. This study addresses how IFN-γ can suppress activation of diabetogenic CD8+ T cells. CD8+ T cells transgenically expressing the diabetogenic AI4 T-cell receptor adoptively transferred disease to otherwise unmanipulated NOD.IFN-γnull , but not standard NOD, mice. AI4 T cells only underwent vigorous intrasplenic proliferation in NOD.IFN-γnull recipients. Disease-protective IFN-γ could be derived from any lymphocyte source and suppressed diabetogenic CD8+ T-cell responses both directly and through an intermediary nonlymphoid cell population. Suppression was not dependent on regulatory T cells, but was associated with increased inhibitory STAT1 to STAT4 expression levels in pathogenic AI4 T cells. Importantly, IFN-γ exposure during activation reduced the cytotoxicity of human-origin type 1 diabetes-relevant autoreactive CD8+ T cells. Collectively, these results indicate that rather than marking the most proinflammatory lymphocytes in diabetes development, IFN-γ production could represent an attempted limitation of pathogenic CD8+ T-cell activation. Thus, great care should be taken when designing possible diabetic intervention approaches modulating IFN-γ production.
- Subjects
TYPE 1 diabetes; ANIMAL models in research; INTERFERONS; INFLAMMATION; CYTOKINES; DIAGNOSIS; RNA metabolism; ANIMAL experimentation; CARRIER proteins; CELL culture; CELL receptors; IMMUNIZATION; IMMUNOLOGY technique; MICE; POLYMERASE chain reaction; RESEARCH funding; SPLEEN; T cells; REVERSE transcriptase polymerase chain reaction
- Publication
Diabetes, 2017, Vol 66, Issue 3, p710
- ISSN
0012-1797
- Publication type
journal article
- DOI
10.2337/db16-0846