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- Title
Association Study Based on the Genome Wide Linkage Results Revealed Two Genes on Chromosome 21 in Type 2 Diabetic Subjects in Japanese.
- Authors
Iwasaki, Naoko; Okamoto, Koji; Nakamura, Shinko; Ogata, Makiko; Tokunaga, Katsushi; Kamatani, Naoyuki; Iwamoto, Yasuhiko
- Abstract
We have previously reported that chromosome 21 showed the significant evidence of linkage with lean cases (BMI2.42; Diabetes 52:209, 2003). For the further analysis, we have done a case-control association study using the same population (one subject from each sib-pair) and normal control subjects by CE-SSCP with pooled DNA samples. Among 106 genes sitting in this lesion, CE-SSCP was worked on 65 genes and we found 9 SNPs that showed significant association with lean cases of type 2 diabetes mellitus. Then we clarified the genotype frequency for these 9 SNPs and found the significant difference in 2 SNPs (p<0.01) between cases and controls. There was only one gene in the linkage block of each SNP. To clarify the causative SNP in the two genes (gene A and gene B), we genotyped the tag SNPs covering the whole genes and all SNPs in exon lesion by direct sequence. We found 3 SNPs that showed the significance in gene A: (p value: AA+Aa v.s. aa+AA v.s. Aa+aa / allele Odds ratio), T3309A (0.641 / 0.00540 / 2.24), C 16184G (0.498 / 0.0004 / 0.39), G41311T (0.597 / 0.0076 / 3.54). And 4 SNPs in gene B: A6991C (0.641 / 0.00885 / 1.65), G9199C (0.641 / 0.00540 / 1.63), G38625A (0.492 / 0.00059 / 1.67), T40111C (0.514 / 0.00487 / 1.53). Our data suggest that these genes would act as a part of genetic cause of lean type 2 diabetic subjects in our population.
- Subjects
JAPAN; HUMAN chromosome 21; TYPE 2 diabetes; GENOMES; PEOPLE with diabetes; GENES
- Publication
Diabetes, 2007, Vol 56, pA645
- ISSN
0012-1797
- Publication type
Article