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- Title
Detecting and Profiling Endogenous RNA G-Quadruplexes in the Human Transcriptome.
- Authors
Zhang, Rongxin; Liu, Yajun; Zhang, Xingxing; Xiao, Ke; Hou, Yue; Liu, Hongde; Sun, Xiao
- Abstract
G-quadruplexes are the non-canonical nucleic acid structures that are preferentially formed in G-rich regions. This structure has been shown to be associated with many biological functions. Regardless of the broad efforts on DNA G-quadruplexes, we still have limited knowledge on RNA G-quadruplexes, especially in a transcriptome-wide manner. Herein, by integrating the DMS-seq and the bioinformatics pipeline, we profiled and depicted the RNA G-quadruplexes in the human transcriptome. The genes that contain RNA G-quadruplexes in their specific regions are significantly related to immune pathways and the COVID-19-related gene sets. Bioinformatics analysis reveals the potential regulatory functions of G-quadruplexes on miRNA targeting at the scale of the whole transcriptome. In addition, the G-quadruplexes are depleted in the putative, not the real, PAS-strong poly(A) sites, which may weaken the possibility of such sites being the real cleaved sites. In brief, our study provides insight into the potential function of RNA G-quadruplexes in post-transcription.
- Subjects
QUADRUPLEX nucleic acids; RNA; NUCLEIC acids; DNA
- Publication
International Journal of Molecular Sciences, 2021, Vol 22, Issue 15, p8012
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms22158012