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- Title
The WRKY Transcription Factor GmWRKY12 Confers Drought and Salt Tolerance in Soybean.
- Authors
Shi, Wen-Yan; Du, Yong-Tao; Ma, Jian; Min, Dong-Hong; Jin, Long-Guo; Chen, Jun; Chen, Ming; Zhou, Yong-Bin; Ma, You-Zhi; Xu, Zhao-Shi; Zhang, Xiao-Hong
- Abstract
WRKYs are important regulators in plant development and stress responses. However, knowledge of this superfamily in soybean is limited. In this study, we characterized the drought- and salt-induced gene GmWRKY12 based on RNA-Seq and qRT-PCR. GmWRKY12, which is 714 bp in length, encoded 237 amino acids and grouped into WRKY II. The promoter region of GmWRKY12 included ABER4, MYB, MYC, GT-1, W-box and DPBF cis-elements, which possibly participate in abscisic acid (ABA), drought and salt stress responses. GmWRKY12 was minimally expressed in different tissues under normal conditions but highly expressed under drought and salt treatments. As a nucleus protein, GmWRKY12 was responsive to drought, salt, ABA and salicylic acid (SA) stresses. Using a transgenic hairy root assay, we further characterized the roles of GmWRKY12 in abiotic stress tolerance. Compared with control (Williams 82), overexpression of GmWRKY12 enhanced drought and salt tolerance, increased proline (Pro) content and decreased malondialdehyde (MDA) content under drought and salt treatment in transgenic soybean seedlings. These results may provide a basis to understand the functions of GmWRKY12 in abiotic stress responses in soybean.
- Subjects
GENETIC transcription; PLANT development; CROP development; SOYBEAN; NUCLEOTIDE sequence
- Publication
International Journal of Molecular Sciences, 2018, Vol 19, Issue 12, p4087
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms19124087