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- Title
RGD-Dependent Mechanotransduction of Suspension Cultured TaxusCell in Response to Shear Stress.
- Authors
Hong Gao; Yan-Wen Gong; Ying-Jin Yuan
- Abstract
Plant cells cultured in bioreactors are strongly influenced by mechanical forces. However, the molecular mechanism of plant cell mechanoreception has maintained unclear. In animal cells, the Arg-Gly-Asp (RGD) motif can be found in proteins of the extracellular matrix. Integrins link the intracellular cytoskeleton of cells with the extracellular matrix by recognizing this RGD motif. Integrin has been demonstrated to function as an apparatus not only for adhesion but also for mechanotransduction. In plant cells, the molecules that mediate the structural continuity between wall and membrane are unknown. Here, we found that synthetic RGD peptide could dramatically reduce the level of phosphorylation of MAPK-like cascades that are activated by shear stress and reduce the alkalinization response, production of reactive oxygen species (ROS) and accumulation of phenolics by Taxuscuspidatacells during shear stress. These results implicate that a RGD recognition system may exist in Taxuscells and play an important role in signal transduction of shear stress. Although the Arabidopsisgenome database shows that the plant seems to lack a homologue of animal integrin, plant cells may use other RGD-binding proteins to recognize the RGD motif. The correlative mechanism is discussed.
- Subjects
PLANT cells &; tissues; CELL culture; BIOREACTORS; MECHANORECEPTORS
- Publication
Biotechnology Progress, 2007, Vol 23, Issue 3, p673
- ISSN
8756-7938
- Publication type
Article