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- Title
Characterization of Arabidopsis thaliana mutant ror- 1 ( roscovitine- resistant) and its utilization in understanding of the role of cytokinin N-glucosylation pathway in plants.
- Authors
Dwivedi, Somya; Vanková, Radomira; Motyka, Vaclav; Herrera, Carmen; Zizkova, Eva; Auer, Carol
- Abstract
Cytokinin analogue roscovitine exhibits a strong inhibitory effect on cytokinin N-glucosylation, one of the most important pathways of cytokinin inactivation in plants. R oscovitine- resistant mutant. ( ror- 1) was isolated using T-DNA tagged lines of Arabidopsis thaliana (L.) Heynh in order to find a gene putatively involved in cytokinin N-glucosylation. The amount of cytokinin N-glucosides of trans-zeatin- and isopentenyladenine-type was elevated by 20% in ror- 1 mutant compared to WT. The cytokinin oxidase/dehydrogenase activity exhibited a mild elevation in ror- 1 compared to WT in basal media. Additionally, ror- 1 plants showed slightly enhanced resistance to exogenously supplied aromatic cytokinins (benzyladenine). Incubation with exogenous cytokinin (5 μM BA for 24 h) resulted in significant up-regulation of ROR-1 gene expression in ror- 1 mutant. In silico analysis showed that ROR- 1 gene encoded for a protein consisting of GRAM (Glycosyltransferases Rab-like GTPase activators and Myotubularins) and C2 domains. Here, we report on the role of ROR- 1 gene in metabolism of bioactive cytokinins in the plants.
- Subjects
ARABIDOPSIS thaliana; CYTOKININS; GLYCOSYLTRANSFERASES; GENE expression; PLANT genetics
- Publication
Plant Growth Regulation, 2010, Vol 61, Issue 3, p231
- ISSN
0167-6903
- Publication type
Article
- DOI
10.1007/s10725-010-9467-8