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- Title
Acridine-Labeled Primers as Tools for the Study of Nonenzymatic RNA Oligomerization.
- Authors
Kurz, Markus; Göbel, Karin; Hartel, Christian; Göbel, Michael W.
- Abstract
Short, dye-labeled oligonucleotides have been used as primers in template-controlled polymerization reactions of RNA. The synthesis of appropriate acridine derivatives and their attachments to nucleic acids is described. In the nonenzymatic oligomerization of 2-methyl-1 H-imidazole-activated guanosine 5′-monophosphate, two observations deserve special notice: ( 1) reaction rates are almost unchanged by variations of the Na+ concentration; ( 2) the conformational type of the primer-template duplex (A vs. B) has considerable influence on the rates and yields of RNA oligomerization. When the incorporation of cytidine was studied in the presence of 1 M Na+ or K+, the process was almost inhibited by quadruplex formation of the oligo-dG template. However, if these cations were omitted, an efficient primer extension could be observed using template concentrations as high as 100 μ M. The chances for nonenzymatic self-replication of RNA thus might be distinctly better than previously assumed.
- Publication
Helvetica Chimica Acta, 1998, Vol 81, Issue 5-8, p1156
- ISSN
0018-019X
- Publication type
Article
- DOI
10.1002/hlca.19980810528