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- Title
Mevalonate production by engineered acetogen biocatalyst during continuous fermentation of syngas or CO/H blend.
- Authors
Kiriukhin, Michael; Tyurin, Michael
- Abstract
Naturally mevalonate-resistant acetogen Clostridium sp. MT1243 produced only 425 mM acetate during syngas fermentation. Using Clostridium sp. MT1243 we engineered biocatalyst selectively producing mevalonate from synthesis gas or CO/H blend. Acetate production and spore formation were eliminated from Clostridium sp. MT1243 using Cre- lox66/ lox71-system. Cell energy released via elimination of phosphotransacetylase, acetate kinase and early stage sporulation genes powered mevalonate accumulation in fermentation broth due to expression of synthetic thiolase, HMG-synthase, and HMG-reductase, three copies of each, integrated using Tn 7-approach. Recombinants produced 145 mM mevalonate in five independent single-step fermentation runs 25 days each in five repeats using syngas blend 60 % CO and 40 % H (v/v) ( p < 0.005). Mevalonate production was 97 mM if only CO/H blend was fed instead of syngas ( p < 0.005). Mevalonate from CO/H blend might serve as a commercial route to mitigate global warming in proportion to CO fermentation scale worldwide.
- Subjects
MEVALONATE kinase; CHEMICAL synthesis; ENZYMES; SYNTHESIS gas; FERMENTATION; THIOLASES
- Publication
Bioprocess & Biosystems Engineering, 2014, Vol 37, Issue 2, p245
- ISSN
1615-7591
- Publication type
Article
- DOI
10.1007/s00449-013-0991-6