We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Developmental stage of transplanted neural progenitor cells influences anatomical and functional outcomes after spinal cord injury in mice.
- Authors
Aceves, Miriam; Tucker, Ashley; Chen, Joseph; Vo, Katie; Moses, Joshua; Amar Kumar, Prakruthi; Thomas, Hannah; Miranda, Diego; Dampf, Gabrielle; Dietz, Valerie; Chang, Matthew; Lukose, Aleena; Jang, Julius; Nadella, Sneha; Gillespie, Tucker; Trevino, Christian; Buxton, Andrew; Pritchard, Anna L.; Green, Peyton; McCreedy, Dylan A.
- Abstract
Neural progenitor cell (NPC) transplantation is a promising therapeutic strategy for replacing lost neurons following spinal cord injury (SCI). However, how graft cellular composition influences regeneration and synaptogenesis of host axon populations, or recovery of motor and sensory functions after SCI, is poorly understood. We transplanted developmentally-restricted spinal cord NPCs, isolated from E11.5-E13.5 mouse embryos, into sites of adult mouse SCI and analyzed graft axon outgrowth, cellular composition, host axon regeneration, and behavior. Earlier-stage grafts exhibited greater axon outgrowth, enrichment for ventral spinal cord interneurons and Group-Z spinal interneurons, and enhanced host 5-HT+ axon regeneration. Later-stage grafts were enriched for late-born dorsal horn interneuronal subtypes and Group-N spinal interneurons, supported more extensive host CGRP+ axon ingrowth, and exacerbated thermal hypersensitivity. Locomotor function was not affected by any type of NPC graft. These findings showcase the role of spinal cord graft cellular composition in determining anatomical and functional outcomes following SCI. In a mouse model of spinal cord injury, the developmental stage of transplanted neural progenitor cells is shown to influence anatomical and behavioral outcomes after spinal cord injury
- Subjects
PROGENITOR cells; SPINAL cord injuries; INTERNEURONS; NEURAL stem cells; FUNCTIONAL status; NERVOUS system regeneration; SPINAL cord; AXONS
- Publication
Communications Biology, 2023, Vol 6, Issue 1, p1
- ISSN
2399-3642
- Publication type
Article
- DOI
10.1038/s42003-023-04893-0