We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
A New Tool for Inducible Gene Expression in Caenorhabditis elegans.
- Authors
Monsalve, Gabriela C.; Yamamoto, Keith R.; Ward, Jordan D.
- Abstract
Controlling protein activity and localization is a key tool in modern biology. Mammalian steroid receptor ligand-binding domain (LBD) fusions have been used in a range of organisms and cell types to inactivate proteins of interest until the cognate steroid ligand is applied. Here, we demonstrate that the glucocorticoid receptor LBD confers ligand-gated control of a heterologous gene expression system (Q system) and the DAF-16 transcription factor in Caenorhabditis elegans. These experiments provide a powerful tool for temporal control of protein activity, and will bolster existing tools used to modulate gene expression and protein activity in this animal.
- Subjects
ANIMAL experimentation; BIOLOGICAL models; CELL receptors; GENE expression; GLUCOCORTICOIDS; NEMATODES; PROTEINS; TEMPORAL lobe; TRANSCRIPTION factors
- Publication
Genetics, 2019, Vol 211, Issue 2, p419
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.118.301705