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- Title
Simultaneous Determination of 14 β-Receptor Agonists Residues in Mutton by High Performance Liquid Chromatography-Tandem Mass Spectrometry ( HPLC-MS/MS).
- Authors
Zhe MENG; Jianhua WANG; Bo LIU; Yuhang GUO; Haoshuang DONG; Pingyang SHAN; Dawei WANG; Yajuan SONG
- Abstract
[Objectives] A high performance liquid chromatography-tandem mass spectrometry {HPLC-MS/MS) method was established for the determination of 14 β-receptor agonist residues in mutton. [Methods] Samples were hydrolyzed by β-glucuronidase and extracted with 5% acetic acid-acetonitrile (1:99, V/V) solution. An Eclipse plus C18 column was used for separation, and the MRM mode was used for qualitative analysis, and the external standard method was used for quantitative analysis of matrix standard solutions. [Results] Under the optimal conditions, the retention time of the 14 kinds of β-receptor agonists ranged from 1. 0 to 9.5 min. When the mass concentration was in the range of 0. 05 - 0. 50 µg/ml, the linear relationship of β-receptor agonists was good, with correlation coefficients (r) ≤ 0.999 2. The detection limits of the method were in the range of 0.04-0.87 µg/kg, and the quantitative limits were in the range of 0.35 -1.86 µg/kg. The average recovery values were in the range of 82.8% -108.9%, with RSDs (n=6) in the range of 1.9% -6.7%. # Conclusions] The method is simple, sensitive, reproducible, accurate, and can be used for simultaneous determination of the 14 kinds of β-receptor agonist residues in mutton.
- Subjects
LIQUID chromatography-mass spectrometry; RF values (Chromatography); DETECTION limit
- Publication
Agricultural Biotechnology (2164-4993), 2023, Vol 12, Issue 5, p55
- ISSN
2164-4993
- Publication type
Article
- DOI
10.19759/j.cnki.2164-4993.2023.05.013