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- Title
A novel nucleotide kinase encoded by gene 1.7 of bacteriophage T7.
- Authors
Tran, Ngoc Q.; Seung-Joo Lee; Richardson, Charles C.; Tabor, Stanley
- Abstract
Gene 1.7 of bacteriophage T7 confers sensitivity of both phage T7 and its host Escherichia coli to dideoxythymidine (ddT). We have purified the product of gene 1.7, gp1.7. It exists in two forms of molecular weight 22 181 and 17 782. Only the C-terminal half of the protein is required to confer ddT sensitivity. We show that gp1.7 catalyses the phosphorylation of dGMP and dTMP to dGDP and dTDP, respectively, by using either GTP, dGTP or dTTP as the phosphate donor. Either form of gp1.7 exhibit identical kinase activity as compared with wild-type gp1.7 that contains a mixture of both forms. The Km of 70 µM and Kcat of 4.3 s−1 for dTMP are similar to those found for E. coli thymidylate kinase. However, unlike the host enzyme, gp1.7 efficiently catalyses the conversion of the chain-terminating dideoxythymidylate (ddTMP) to ddTDP. This finding explains the sensitivity of phage T7 but not E. coli to exogenous ddT. Gp1.7 is unusual in that it has no sequence homology to any known nucleotide kinase, it has no identifiable nucleotide-binding motif and its activity is independent of added metal ions. When coupled with nucleoside diphosphate kinase, gp1.7 exponentially converts dTMP to dTTP.
- Subjects
NUCLEOTIDES; BACTERIOPHAGES; HOSTS (Biology); ESCHERICHIA coli; MOLECULAR microbiology; GENETICS
- Publication
Molecular Microbiology, 2010, Vol 77, Issue 2, p492
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/j.1365-2958.2010.07221.x