We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Evaluation of the prognostic impact of pyknotic nuclei in prostate cancer cells: A retrospective cohort study.
- Authors
Diop, Mame-Kany; Ouellet, Véronique; Benzerdjeb, Nazim; Latour, Mathieu; Albadine, Roula; Aprikian, Armen G.; Lacombe, Louis; Fleshner, Neil E.; Gleave, Martin E.; Mes-Masson, Anne-Marie; Saad, Fred; Trudel, Dominique
- Abstract
Objective: Despite the early diagnosis of non-metastatic prostate cancer (PCa), a large variation in survival rate is observed. It is imperative to find new prognostic factors to better guide the treatment of PCa. In a preliminary study we found that a higher percentage of cells with pyknotic nuclei was observed in the more aggressive cancers. Our goal is to assess the prognostic potential of pyknotic nuclei in PCa. Method: Stained with hematoxylin and eosin, the tissue microarrays of a test cohort of 250 patients, and a validation cohort of 1262 patients, were analyzed to visually estimate the amount of cells with pyknotic nuclei among the cancer cells. Patients were classified into 2 categories according to the estimated percentage of pyknotic nuclei. Data and results: More pyknotic nuclei were observed in patients with positive surgical margins (p=0.001), lymph node metastases (p=0.004), extraprostatic extension (p=0.018), and seminal vesicle involvement (p=0.024). In addition, the survival rate without biochemical recurrence (BCR) is shorter in patients with a higher amount of pyknotic nuclei and a Gleason score (GS) of 3+4 (p=0.041). From a further study on whole slides (N=5), the distribution of pyknotic nuclei was not always homogeneous in the tumour. Conclusions: Pyknotic nuclei are linked to adverse prognostic factors and associated with poor prognosis in patients with a GS of 3+4. However, because of the heterogeneous distribution of pyknotic nuclei across the complete tumour, quantifying pyknotic nuclei in whole sections is necessary.
- Subjects
CELL nuclei; PROSTATE cancer; CANCER diagnosis; MICROARRAY technology; EOSIN; CANCER relapse
- Publication
Canadian Journal of Pathology, 2018, Vol 10, p41
- ISSN
1918-915X
- Publication type
Article