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- Title
Charge-Induced Unzipping of Isolated Proteins to a Defined Secondary Structure.
- Authors
González Flórez, Ana Isabel; Mucha, Eike; Ahn, Doo‐Sik; Gewinner, Sandy; Schöllkopf, Wieland; Pagel, Kevin; von Helden, Gert
- Abstract
Here we present a combined experimental and theoretical study on the secondary structure of isolated proteins as a function of charge state. In infrared spectra of the proteins ubiquitin and cytochrome c, amide I (C=O stretch) and amide II (N-H bend) bands can be found at positions that are typical for condensed-phase proteins. For high charge states a new band appears, substantially red-shifted from the amide II band observed at lower charge states. The observations are interpreted in terms of Coulomb-driven transitions in secondary structures from mostly helical to extended C5-type hydrogen-bonded structures. Support for this interpretation comes from simple energy considerations as well as from quantum chemical calculations on model peptides. This transition in secondary structure is most likely universal for isolated proteins that occur in mass spectrometric experiments.
- Subjects
PROTEIN structure; UBIQUITIN structure; CYTOCHROME c; AMIDES; CONDENSED matter
- Publication
Angewandte Chemie International Edition, 2016, Vol 55, Issue 10, p3295
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201510983