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- Title
Development, characterization, and comparative analysis of polymorphism at common bean SSR loci isolated from genic and genomic sources.
- Authors
Hanai, Luiz Ricardo; De Campos, Tatiana; Camargo, Luis Eduardo Aranha; Benchimol, Luciana Lasry; De Souza, Anete Pereira; Melotto, Maeli; Carbonell, Sérgio Augusto Moraes; Chioratto, Alisson Fernando; Consoli, Luciano; Formighieri, Eduardo Fernando; Siqueira, Marcos Vinícius Bohrer Monteiro; Siu Mui Tsai; Vieira, Maria Lucia Carneiro
- Abstract
Microsatellites or SSRs (single sequence repeats) have been used to construct and integrate genetic maps in crop species, including Phaseolus vulgaris. In the present study, 3 cDNA libraries generated by the Bean EST project (<A>http://lgm.esalq.usp.br/BEST/</A>), comprising a unigene collection of 3126 sequences and a genomic microsatellite-enriched library, were analyzed for the presence of SSRs. A total of 219 expressed sequence tags (ESTs) were found to carry 240 SSRs (named EST-SSR), whereas 714 genomic sequences contained 471 SSRs (named genomic-SSR). A subset of 80 SSRs, 40 EST-SSRs, and 40 genomic-SSRs were evaluated for molecular polymorphism in 23 genotypes of cultivated beans from the Mesoamerican and Andean genetic pools, including Brazilian cultivars and 2 related species. Of the common bean genotypes, 31 EST-SSR loci were polymorphic, yielding 2–12 alleles as compared with 26 polymorphic genomic-SSRs, accounting for 2–7 alleles. Cluster analysis from data using both genic and genomic-SSR revealed a clear separation between Andean and Mesoamerican beans. The usefulness of these loci for distinguishing bean genotypes and genetic mapping is discussed.
- Subjects
GENETIC polymorphisms; MICROSATELLITE repeats; GENE mapping; BEAN genetics; ANTISENSE DNA; PLANT genomes
- Publication
Genome, 2007, Vol 50, Issue 3, p266
- ISSN
0831-2796
- Publication type
Article
- DOI
10.1139/G07-007