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- Title
Determination of chemical composition and anti-fungal activities of aromatic water of Zataria multiflora Boiss.
- Authors
Yazdanpanah, Maryam; Zomorodian, Kamiar; Mehrabani, Davood; Yazdanpanah, Samira; PakShir, Keyvan; Monfared, Ali Arabi; Iraji, Aida
- Abstract
Introduction: Candida species, as a part of normal flora of mucocutaneous surfaces, may cause wide range of clinical symptom from superficial infection to mucocutaneous or visceral candidiasis. These yeasts may also cause upper gastrointestinal especially among those with imbalance normal flora or compromised immune system. Regarding universal emergence of antifungalresistant Candida, there is growing tendency in finding novel antifungal agents especially from natural resources. Among which, aromatic waters (AW) distilled from medicinal plant containing essential oils with known antimicrobial properties might be a good candidate. The aim of this study was to determined in vitro and in vivo antifungal activities of Zataria multiflora Boiss AW against Candida species. Materials and Methods: The chemical composition of the essential oil from AW Zataria multiflora analyzed by gas chromatography-mass spectrometry (GCMS). The antimicrobial activity of the essential oil against Candida species was evaluated by broth micro-dilution as per the Clinical and Laboratory Standards Institute (CLSI) methods. Moreover, biofilm formation inhibition and antioxidant activity of the AW was measured by using a XTT reduction and DPPH methods, respectively. Experimental activity of the AW in the prevention or treatment of GI candidiasis was also evaluated in animal model by both culture and histopathological methods. Result: GC-MS analysis revealed that the major constituents of the essential oil of AW were thymol (40.67%) and carvacrol (46.56%). The Zataria multiflora AW exhibited antimicrobial activity against all tested yeasts with MICs in the range of 0.25-0. 5V/V. In addition, the EO inhibited the biofilm formation of Candida albicans at concentration up to 0.5V/V (70%). The AW significantly decreased the CFUs in mice receiving AW in comparison with those of control group. Similarly, histopathological analyses showed that Candida colonization decline in mice following administration of AW of Zataria multiflora in therapeutic trial. Conclusion: The considerable antifungal activity of the AW against the examined Candida species might be related to high concentration of phenolic monoterpens in the EO distilled from AW. In addition to considerable antimicrobial effects of the AW, antioxidant activity of the AW attributed to inducing the healing process of tissue necrosis found in mice treated with AW in comparison to the controls. Considering wide range of antifungal activities of the examined AW, it might have potential to be used in the management of alimentary candidiasis or as mouthwash or other pharmaceutical products.
- Subjects
IRAN; CONFERENCES &; conventions; FUNGI; MEDICINAL plants
- Publication
Current Medical Mycology, 2018, Vol 4, p160
- ISSN
2423-3439
- Publication type
Article
- DOI
10.18502/cmm.4.S1.2018.180