We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
IRS2 is a candidate driver oncogene on 13q34 in colorectal cancer.
- Authors
Day, Elizabeth; Poulogiannis, George; McCaughan, Frank; Mulholland, Shani; Arends, Mark J.; Ibrahim, Ashraf E. K.; Dear, Paul H.
- Abstract
Copy number alterations are frequently found in colorectal cancer ( CRC), and recurrent gains or losses are likely to correspond to regions harbouring genes that promote or impede carcinogenesis respectively. Gain of chromosome 13q is common in CRC but, because the region of gain is frequently large, identification of the driver gene(s) has hitherto proved difficult. We used array comparative genomic hybridization to analyse 124 primary CRCs, demonstrating that 13q34 is a region of gain in 35% of CRCs, with focal gains in 4% and amplification in a further 1.6% of cases. To reduce the number of potential driver genes to consider, it was necessary to refine the boundaries of the narrowest copy number changes seen in this series and hence define the minimal copy region ( MCR). This was performed using molecular copy-number counting, identifying IRS2 as the only complete gene, and therefore the likely driver oncogene, within the refined MCR. Analysis of available colorectal neoplasia data sets confirmed IRS2 gene gain as a common event. Furthermore, IRS2 protein and m RNA expression in colorectal neoplasia was assessed and was positively correlated with progression from normal through adenoma to carcinoma. In functional in vitro experiments, we demonstrate that deregulated expression of IRS2 activates the oncogenic PI3 kinase pathway and increases cell adhesion, both characteristics of invasive CRC cells. Together, these data identify IRS2 as a likely driver oncogene in the prevalent 13q34 region of gain/amplification and suggest that IRS2 over-expression may provide an additional mechanism of PI3 kinase pathway activation in CRC.
- Subjects
COLON cancer; ONCOGENES; INSULIN receptors; CARCINOGENESIS; GENE expression; CHROMOSOMES; MESSENGER RNA
- Publication
International Journal of Experimental Pathology, 2013, Vol 94, Issue 3, p203
- ISSN
0959-9673
- Publication type
Article
- DOI
10.1111/iep.12021