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- Title
Human ABO gene transcriptional regulation.
- Authors
Kominato, Yoshihiko; Sano, Rie; Takahashi, Yoichiro; Hayakawa, Akira; Ogasawara, Kenichi
- Abstract
ABBREVIATIONS ABOi ABO-incompatible AML acute myeloid leukemia CGIs CpG islands ChIP chromatin immunoprecipitation DHSs DNase I-hypersensitive sites EMSAs electrophoretic mobility shift assays HGVS Human Genome Variation Society MDS myelodysplastic syndrome SSP single specific primer TFs transcription factors vWF von Willebrand factor The ABO blood group system, discovered by Karl Landsteiner in 1900,[1] is fundamental to the safety of blood transfusion, which requires identification of weak phenotypes or subgroups. Thus, it remains controversial whether I ABO i transcription is influenced by the CCAAT-binding factor/NF-Y enhancer region in erythroid cells.[[36], [52]] Distal promoter of ABO At the 5' end of the CGI involving the I ABO i proximal promoter in cultured cells expressing I ABO i , an alternative starting Exon 1a comprising 27 base pairs was found by 5'-RACE (Fig. The region proximal to the proximal ABO promoter The plasmid-based reporter assays demonstrated a negative regulatory element just upstream from the proximal I ABO i promoter in KATOIII cells and HEL cells,[54] suggesting that I ABO i transcription is regulated by negative elements in the -307 to -150 region from the translation start site. Cell differentiation-specific regulation In vitro erythroid differentiation of CD34 SP + sp cells and AC133 SP - sp CD34 SP + sp cells from peripheral blood mononuclear cells indicated that I ABO i was expressed at an early stage and disappeared later,[[35], [42]] and that the period when I ABO i was expressed at a higher level preceded that of I FUT1 i expression (Fig.).
- Subjects
BLOOD group incompatibility; GENETIC regulation; BLADDER cancer; HUMAN genes; ABO blood group system; BLOOD group antigens; CELL surface antigens
- Publication
Transfusion, 2020, Vol 60, Issue 4, p860
- ISSN
0041-1132
- Publication type
journal article
- DOI
10.1111/trf.15760