We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Cytotoxicity of natural extract from Tegillarca granosa on ovarian cancer cells is mediated by multiple molecules.
- Authors
Gao, Ming-Qing; Han, Yan-Tao; Zhu, Li; Chen, Shou-Guo; Hong, Zhen-Yu; Wang, Chun-Bo
- Abstract
Purpose: To determine the cellular and molecular mechanism of cytotoxicity induced by Haishengsu (HSS), nature extract from Tegillarca granosa, toward human ovarian cancer cell lines SKOV-3 and OVCAR-3. Methods: The cytotoxic effects of HSS on two ovarian cancer cell lines were tested by XTT assay. Cell apoptosis and cell cycle arrest induced by HSS were demonstrated by DNA ladder assay and flow cytometric analysis, respectively. RT-PCR or flow cytometric analysis was used to investigate the expression of bcl-2, caspase-3, p53, β-catenin, E-cadherin, CD24, and CD44. Results: Continuous exposure to HSS for 48 h produced cytotoxic effects on both cell lines in a concentration dependent manner, which was accompanied by apoptosis and cell cycle arrest. Apoptosis associated gene bcl-2 and caspase-3, tumor metastasis associated gene β-catenin, but not E-cadherin, and CD24, but not CD44, were involved in the effect of growth inhibition induced by HSS. Although p53 mediated apoptosis induced by HSS in OVCAR-3 cells, it was not required in SKOV-3 cells. Conclusion: HSS has a potential cytotoxic effect on human ovarian cancer cells, which was mediated by multiple signal molecules including bcl-2, caspase-3, β-catenin, and CD24. These findings will provide a theoretical basis for HSS's potential clinical application as a novel marine anticancer agent.
- Subjects
CELLULAR mechanics; CELL-mediated cytotoxicity; ISOHORMONES; APOPTOSIS; OVARIAN cancer; ANTINEOPLASTIC agents
- Publication
Clinical & Investigative Medicine, 2009, Vol 32, Issue 5, pE368
- ISSN
0147-958X
- Publication type
Article
- DOI
10.25011/cim.v32i5.6925