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- Title
Glutamate-mediated overexpression of CD38 in astrocytes cultured with neurones.
- Authors
Bruzzone, Santina; Verderio, Claudia; Schenk, Ursula; Fedele, Ernesto; Zocchi, Elena; Matteoli, Michela; De Flora, Antonio
- Abstract
Recently, a new system of astrocyte–neurone glutamatergic signalling has been identified. It is started in astrocytes by ectocellular, CD38-catalysed conversion of NAD+ to the calcium mobilizer cyclic ADP-ribose (cADPR). This is then pumped by CD38 itself into the cytosol where the resulting free intracellular Ca2+ concentration [Ca2+i transients elicit an increased release of glutamate, which can induce an enhanced Ca2+ response in neighbouring neurones. Here, we demonstrate that co-culture of either cortical or hippocampal astrocytes with neurones results in a significant overexpression of astrocyte CD38 both on the plasma membrane and intracellularly. The causal role of neurone-released glutamate in inducing overexpression of astrocyte CD38 is demonstrated by two observations: first, in the absence of neurones, induction of CD38 in pure astrocyte cultures can be obtained with glutamate and second, it can be prevented in co-cultures by glutamate receptor antagonists. The neuronal glutamate-mediated effect of neurones on astrocyte CD38 expression is paralleled by increased intracellular cADPR and [Ca2+i levels, both findings indicating functionality of overexpressed CD38. These results reveal a new neurone-to-astrocyte glutamatergic signalling based on the CD38/cADPR system, which affects the [Ca2+i in both cell types, adding further complexity to the bi-directional patterns of communication between astrocytes and neurones.
- Subjects
ASTROCYTES; NEUROGLIA; ADENOSINE diphosphate; ENZYMES; CALCIUM in the body; NEUROCHEMISTRY
- Publication
Journal of Neurochemistry, 2004, Vol 89, Issue 1, p264
- ISSN
0022-3042
- Publication type
Article
- DOI
10.1111/j.1471-4159.2003.02326.x