We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Impact of a high-fat meal on assessment of clopidogrel-induced platelet inhibition in healthy subjects.
- Authors
Dobesh, Paul P.; Urban, Jamela F.; Shurmur, Scott W.; Oestreich, Julie H.
- Abstract
Background Ideal conditions for platelet reactivity testing are critical for optimal selection of a P2Y12 inhibitor. Data are inconsistent regarding the impact of high-fat meals on test assessment. Methods Participants included 12 healthy subjects not taking antiplatelet drugs after a 12-hour fast. After baseline assessment, subjects were given a 600 mg dose of clopidogrel. Four hours later, maximum platelet inhibition was tested in the fasting state by light transmission aggregometry (LTA), VerifyNow P2Y12, vasodilator-stimulated phosphoprotein (VASP), and whole blood aggregometry (WBA). Subjects were then provided a high-fat meal, and platelet function was evaluated two hours later. Change in measured platelet aggregation by LTA was the primary endpoint of the study. The Wilcoxon Rank Sum test was used to compare the change in platelet reactivity between fasting and non-fasting conditions. The Spearman rho (ρ) correlation coefficient was used to evaluate the association between fasting platelet reactivity and the change following a high-fat meal. Results No significant change occurred in maximal light transmission, as assessed by LTA with 5 μM ADP (p = 0.15) and with 20 μM ADP (p = 0.07). There was a significant change in the area under the curve with 5 μM ADP (p = 0.03) but not with 20 μM ADP (p = 0.18). Although there was no significant change with the VerifyNow P2Y12 assay (p = 0.16), the change was correlated with the initial fasting value (Spearman's rho p = 0.008). The VASP assay and WBA varied minimally. Conclusion The high-fat meal did not significantly alter platelet function assessment of commonly used platelet function tests. Greater intra-subject variability existed for the optically-dependent compared with non-optically dependent tests.
- Subjects
BLOOD platelets; CLOPIDOGREL; LIPID analysis; BIOLOGICAL assay; FAT content of food; SERIAL publications; STATISTICS; THROMBOSIS; DATA analysis; PATIENT selection; DATA analysis software; PHYSIOLOGY
- Publication
Thrombosis Journal, 2015, Vol 13, Issue 1, p1
- ISSN
1477-9560
- Publication type
Article
- DOI
10.1186/s12959-014-0033-x