We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
NADase as a target molecule of in vivo suppression of the toxicity in the invasive M-1 group A Streptococcal isolates.
- Authors
Tatsuno, Ichiro; Isaka, Masanori; Minami, Masaaki; Hasegawa, Tadao
- Abstract
Background: NAD-glycohydrolase (NADase) secreted by M-1 group A streptococcal (GAS) isolates are suspected as one of the virulence factors to cause severe invasive disease including streptococcal toxic shock-like syndrome (STSS). M-1 GAS strains were divided into three groups based on NADase activity: high activity, low activity and no activity in our previous report. Results: The representative high activity isolates taken from STSS patients showed higher virulence compared with isolates from the low activity group, when used to infect mice. The knockout mutant of the nga gene, which encodes NADase also showed reduced virulence in a mouse infection study. The cloned nga gene was able to significantly complement the lost virulence. In addition, the solution containing purified recombinant IFS, which is an inhibitor of NADase, partially rescued mice infected with S. pyogenes. Conclusions: These results indicate that NADase is important for the virulence of S. pyogenes in vivo and is the potential target to suppress the virulence.
- Subjects
IN vivo toxicity testing; STREPTOCOCCAL diseases; MICROBIAL virulence; GENES; BACTERIAL diseases
- Publication
BMC Microbiology, 2010, Vol 10, p144
- ISSN
1471-2180
- Publication type
Article
- DOI
10.1186/1471-2180-10-144