We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
The 5′ untranslated region of potato SBgLR gene contributes to pollen-specific expression.
- Authors
Chang, Yujie; Yan, Min; Yu, Jingjuan; Zhu, Dengyun; Zhao, Qian
- Abstract
Main conclusion : The 5′UTR of SBgLR enhances gene expression by regulating both its transcription and translation. SBgLR ( Solanum tuberosum genomic lysine rich) is a pollen-specific gene in Solanum tuberosum that encodes a microtubule-associated protein. The region from −85 to +180 (transcription start site at +1) was determined to be critical for specific expression in pollen grains. Transient and stable expression assays showed that the 5′UTR (from +1 to +184) enhanced gene expression in all detected tissues of transgenic tobacco. Deletion analysis demonstrated that the secondary structure of the 5′UTR had no effect on pollen-specific SBgLR expression, while the region from +31 to +60 was crucial. Further investigation indicated that mRNA expression was slightly decreased when the +31 to +60 region was deleted, but the mRNA decay rate remained unchanged. Mutation analysis also confirmed that the pollen-specific element TTTCT, located at +37, played an important role in pollen-specific expression. Using yeast one-hybrid screening, we isolated a DNA-binding with one finger (Dof) protein gene ( StDof23) and an AT-hook motif nuclear-localized (AHL) protein gene ( StAHL) from potato pollen. Further investigation indicated that StDof23 interacted with and positively regulated the +31 to +60 region; moreover, StAHL interacted with and negatively regulated the −49 to +60 region. These results demonstrate that the 5′UTR not only enhanced gene expression but also altered the tissue-specific expression pattern by regulating both transcription and translation.
- Subjects
POTATOES; PLANT genes; POLLEN; GENE expression; TOBACCO
- Publication
Planta: An International Journal of Plant Biology, 2017, Vol 246, Issue 3, p389
- ISSN
0032-0935
- Publication type
Article
- DOI
10.1007/s00425-017-2695-7