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- Title
Inorganic polyphosphate differentiates human mesenchymal stem cells into osteoblastic cells.
- Authors
Morimoto, Daiki; Tomita, Tetsuya; Kuroda, Shoko; Higuchi, Chikahisa; Kato, Shinichi; Shiba, Toshikazu; Nakagami, Hironori; Morishita, Ryuichi; Yoshikawa, Hideki
- Abstract
The existence of inorganic polyphosphates [poly(P)] in human cells has been demonstrated. In osteoblasts, it is suggested that the concentration of cellular poly(P) is relatively high. In this study, we examined whether poly(P) accelerates the differentiation of human mesenchymal stem cells (hMSCs) from patients with osteoarthritis (OA) and rheumatoid arthritis (RA) into osteoblastic cells. Alkaline phosphatase (ALP) activity was induced by poly(P) in hMSCs from both OA and RA. In Alizarin Red S and osteocalcin EIA, there was a significant difference between the control and poly(P) group. In real-time PCR, there was a significant difference in ALP, collagen type 1A, osteocalcin, and bone sialoprotein between the control and poly(P) group. Our findings suggest that poly(P) have the potent role of differentiating hMSCs into osteoblastic cells at the early and later stages of osteoblastic differentiation.
- Subjects
POLYPHOSPHATES; OSTEOARTHRITIS; MESENCHYMAL stem cells; RHEUMATOID arthritis; EXTRACELLULAR matrix proteins; ALKALINE phosphatase; CELL culture; CELL differentiation; CONNECTIVE tissue cells; IMMUNOENZYME technique; PHOSPHATES; POLYMERASE chain reaction; OSTEOBLASTS; OSTEOCALCIN
- Publication
Journal of Bone & Mineral Metabolism, 2010, Vol 28, Issue 4, p418
- ISSN
0914-8779
- Publication type
journal article
- DOI
10.1007/s00774-010-0157-4