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- Title
Fed-batch strategies using butyrate for high cell density cultivation of Pseudomonas putida and its use as a biocatalyst.
- Authors
Cerrone, Federico; Duane, Gearoid; Casey, Eoin; Davis, Reeta; Belton, Ian; Kenny, Shane; Guzik, Maciej; Woods, Trevor; Babu, Ramesh; O'Connor, Kevin
- Abstract
A mathematically based fed-batch bioprocess demonstrated the suitability of using a relatively cheap and renewable substrate (butyric acid) for Pseudomonas putida CA-3 high cell density cultivation. Butyric acid fine-tuned addition is critical to extend the fermentation run and avoid oxygen consumption while maximising the biomass volumetric productivity. A conservative submaximal growth rate (μ of 0.25 h) achieved 71.3 g L of biomass after 42 h of fed-batch growth. When a more ambitious feed rate was supplied in order to match a μ of 0.35 h, the volumetric productivity was increased to 2.0 g L h, corresponding to a run of 25 h and 50 g L of biomass. Both results represent the highest biomass and the best biomass volumetric productivity with butyrate as a sole carbon source. However, medium chain length polyhydroxyalkanoate (mcl-PHA) accumulation with butyrate grown cells is low (4 %). To achieve a higher mcl-PHA volumetric productivity, decanoate was supplied to butyrate grown cells. This strategy resulted in a PHA volumetric productivity of 4.57 g L h in the PHA production phase and 1.63 g L hover the lifetime of the fermentation, with a maximum mcl-PHA accumulation of 65 % of the cell dry weight.
- Subjects
PSEUDOMONAS putida; BUTYRATES; BACTERIAL cells; BACTERIAL enzymes; BACTERIAL cultures; FERMENTATION
- Publication
Applied Microbiology & Biotechnology, 2014, Vol 98, Issue 22, p9217
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-014-5989-8