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- Title
Genotyping of human bocavirus using a restriction length polymorphism.
- Authors
Vanessa Ditt; Sergei Viazov; Ramona Tillmann; Verena Schildgen; Oliver Schildgen
- Abstract
Abstract  Sequencing analysis of the isolates of a recently identified pathogen associated with respiratory infections, human bocavirus (HBoV), allowed for identification of two virus genotypes of the virus. In the current article a new method for a simple and fast differentiation of HBoV genotypes in clinical materials is described. The test includes an amplification of a 309 bp fragment of VP1/VP2 gene of HBoV from nasopharyngeal aspirates with a subsequent incubation of a PCR mix with the BstAPI endonuclease. Upon such a digestion, the DNA fragment derived from the genotype I HBoV isolates forms two fragments of 150 and 159 bp, while that obtained from genotype 2 isolates remains unrestricted. The developed technique may be used in epidemiological studies of HBoV infection and analysis of the potential differences in biological characteristics of HboV genotypes.
- Subjects
GENETIC polymorphisms; GENETIC research; NUCLEIC acids; VIRUSES
- Publication
Virus Genes, 2008, Vol 36, Issue 1, p67
- ISSN
0920-8569
- Publication type
Article
- DOI
10.1007/s11262-007-0182-0